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bdna signal amplification scheme  (Chiron Corporation)

 
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    Structured Review

    Chiron Corporation bdna signal amplification scheme
    FISH of HBV RNA and DNA in a hepatoma cell line supporting HBV replication. HepG2-NTCP ( A ) and HepAD38 ( B – D ) cells, which are either maintained in doxycycline-supplemented medium ( C ) or doxycycline-free medium, were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After <t>bDNA</t> <t>amplification,</t> fluorophore-conjugated label probe was applied and images were acquired with a Deltavision epifluorescence microscope ( A – C ) and Nikon 3D-SIM microscope (Tokyo, Japan) ( D ). Scale bar, 4 μm.
    Bdna Signal Amplification Scheme, supplied by Chiron Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bdna signal amplification scheme/product/Chiron Corporation
    Average 90 stars, based on 1 article reviews
    bdna signal amplification scheme - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Establishment of a fluorescent in situ hybridization assay for imaging hepatitis B virus nucleic acids in cell culture models"

    Article Title: Establishment of a fluorescent in situ hybridization assay for imaging hepatitis B virus nucleic acids in cell culture models

    Journal: Emerging Microbes & Infections

    doi: 10.1038/emi.2017.84

    FISH of HBV RNA and DNA in a hepatoma cell line supporting HBV replication. HepG2-NTCP ( A ) and HepAD38 ( B – D ) cells, which are either maintained in doxycycline-supplemented medium ( C ) or doxycycline-free medium, were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After bDNA amplification, fluorophore-conjugated label probe was applied and images were acquired with a Deltavision epifluorescence microscope ( A – C ) and Nikon 3D-SIM microscope (Tokyo, Japan) ( D ). Scale bar, 4 μm.
    Figure Legend Snippet: FISH of HBV RNA and DNA in a hepatoma cell line supporting HBV replication. HepG2-NTCP ( A ) and HepAD38 ( B – D ) cells, which are either maintained in doxycycline-supplemented medium ( C ) or doxycycline-free medium, were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After bDNA amplification, fluorophore-conjugated label probe was applied and images were acquired with a Deltavision epifluorescence microscope ( A – C ) and Nikon 3D-SIM microscope (Tokyo, Japan) ( D ). Scale bar, 4 μm.

    Techniques Used: Hybridization, Amplification, Microscopy

    STORM imaging of HBV DNA. ( A ) HepAD38 cells cultured in doxycycline-free medium were fixed and permeabilized followed by hybridization with Probe set 2. After bDNA amplification, fluorophore-conjugated label probe was applied and images were reconstructed from stochastic images as described in the Materials and Methods section. Cell nuclei were imaged after STORM imaging using conventional fluorescence microscopy and merged. Scale bar, 5 μm. The surface areas ( B ) and circularity ( C ) of detected puncta in epifluorescence, 3D-SIM and 3D-STORM platforms were compared.
    Figure Legend Snippet: STORM imaging of HBV DNA. ( A ) HepAD38 cells cultured in doxycycline-free medium were fixed and permeabilized followed by hybridization with Probe set 2. After bDNA amplification, fluorophore-conjugated label probe was applied and images were reconstructed from stochastic images as described in the Materials and Methods section. Cell nuclei were imaged after STORM imaging using conventional fluorescence microscopy and merged. Scale bar, 5 μm. The surface areas ( B ) and circularity ( C ) of detected puncta in epifluorescence, 3D-SIM and 3D-STORM platforms were compared.

    Techniques Used: Imaging, Cell Culture, Hybridization, Amplification, Fluorescence, Microscopy

    Detection of HBV RNA and DNA in HBV virus-infected HepG2-NTCP cells. HepG2-NTCP cells were either mock infected ( A ) or infected with 100 × concentrated HepAD38 cell supernatant ( B – D ). Seven days after infection, cells were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After bDNA amplification, fluorophore-conjugated label probe was applied and images were acquired using a Deltavision epifluorescence microscope with a × 100 oil-immersion objective. Image ( D ) was an enlarged area in the rectangle region of ( C ). Scale bar, 4 μm.
    Figure Legend Snippet: Detection of HBV RNA and DNA in HBV virus-infected HepG2-NTCP cells. HepG2-NTCP cells were either mock infected ( A ) or infected with 100 × concentrated HepAD38 cell supernatant ( B – D ). Seven days after infection, cells were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After bDNA amplification, fluorophore-conjugated label probe was applied and images were acquired using a Deltavision epifluorescence microscope with a × 100 oil-immersion objective. Image ( D ) was an enlarged area in the rectangle region of ( C ). Scale bar, 4 μm.

    Techniques Used: Virus, Infection, Hybridization, Amplification, Microscopy



    Similar Products

    bdna signal amplification scheme  (Chiron Corporation)
    90
    Chiron Corporation bdna signal amplification scheme
    FISH of HBV RNA and DNA in a hepatoma cell line supporting HBV replication. HepG2-NTCP ( A ) and HepAD38 ( B – D ) cells, which are either maintained in doxycycline-supplemented medium ( C ) or doxycycline-free medium, were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After <t>bDNA</t> <t>amplification,</t> fluorophore-conjugated label probe was applied and images were acquired with a Deltavision epifluorescence microscope ( A – C ) and Nikon 3D-SIM microscope (Tokyo, Japan) ( D ). Scale bar, 4 μm.
    Bdna Signal Amplification Scheme, supplied by Chiron Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bdna signal amplification scheme/product/Chiron Corporation
    Average 90 stars, based on 1 article reviews
    bdna signal amplification scheme - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    FISH of HBV RNA and DNA in a hepatoma cell line supporting HBV replication. HepG2-NTCP ( A ) and HepAD38 ( B – D ) cells, which are either maintained in doxycycline-supplemented medium ( C ) or doxycycline-free medium, were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After bDNA amplification, fluorophore-conjugated label probe was applied and images were acquired with a Deltavision epifluorescence microscope ( A – C ) and Nikon 3D-SIM microscope (Tokyo, Japan) ( D ). Scale bar, 4 μm.

    Journal: Emerging Microbes & Infections

    Article Title: Establishment of a fluorescent in situ hybridization assay for imaging hepatitis B virus nucleic acids in cell culture models

    doi: 10.1038/emi.2017.84

    Figure Lengend Snippet: FISH of HBV RNA and DNA in a hepatoma cell line supporting HBV replication. HepG2-NTCP ( A ) and HepAD38 ( B – D ) cells, which are either maintained in doxycycline-supplemented medium ( C ) or doxycycline-free medium, were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After bDNA amplification, fluorophore-conjugated label probe was applied and images were acquired with a Deltavision epifluorescence microscope ( A – C ) and Nikon 3D-SIM microscope (Tokyo, Japan) ( D ). Scale bar, 4 μm.

    Article Snippet: The ViewRNA technology uses the bDNA signal amplification scheme, which was initially developed by the Chiron Corporation to quantify the HIV and HCV viral loads., , , The bDNA scheme can also be used to quantify DNA molecules, such as HBV DNA, with a detection limit of 2000 copies.

    Techniques: Hybridization, Amplification, Microscopy

    STORM imaging of HBV DNA. ( A ) HepAD38 cells cultured in doxycycline-free medium were fixed and permeabilized followed by hybridization with Probe set 2. After bDNA amplification, fluorophore-conjugated label probe was applied and images were reconstructed from stochastic images as described in the Materials and Methods section. Cell nuclei were imaged after STORM imaging using conventional fluorescence microscopy and merged. Scale bar, 5 μm. The surface areas ( B ) and circularity ( C ) of detected puncta in epifluorescence, 3D-SIM and 3D-STORM platforms were compared.

    Journal: Emerging Microbes & Infections

    Article Title: Establishment of a fluorescent in situ hybridization assay for imaging hepatitis B virus nucleic acids in cell culture models

    doi: 10.1038/emi.2017.84

    Figure Lengend Snippet: STORM imaging of HBV DNA. ( A ) HepAD38 cells cultured in doxycycline-free medium were fixed and permeabilized followed by hybridization with Probe set 2. After bDNA amplification, fluorophore-conjugated label probe was applied and images were reconstructed from stochastic images as described in the Materials and Methods section. Cell nuclei were imaged after STORM imaging using conventional fluorescence microscopy and merged. Scale bar, 5 μm. The surface areas ( B ) and circularity ( C ) of detected puncta in epifluorescence, 3D-SIM and 3D-STORM platforms were compared.

    Article Snippet: The ViewRNA technology uses the bDNA signal amplification scheme, which was initially developed by the Chiron Corporation to quantify the HIV and HCV viral loads., , , The bDNA scheme can also be used to quantify DNA molecules, such as HBV DNA, with a detection limit of 2000 copies.

    Techniques: Imaging, Cell Culture, Hybridization, Amplification, Fluorescence, Microscopy

    Detection of HBV RNA and DNA in HBV virus-infected HepG2-NTCP cells. HepG2-NTCP cells were either mock infected ( A ) or infected with 100 × concentrated HepAD38 cell supernatant ( B – D ). Seven days after infection, cells were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After bDNA amplification, fluorophore-conjugated label probe was applied and images were acquired using a Deltavision epifluorescence microscope with a × 100 oil-immersion objective. Image ( D ) was an enlarged area in the rectangle region of ( C ). Scale bar, 4 μm.

    Journal: Emerging Microbes & Infections

    Article Title: Establishment of a fluorescent in situ hybridization assay for imaging hepatitis B virus nucleic acids in cell culture models

    doi: 10.1038/emi.2017.84

    Figure Lengend Snippet: Detection of HBV RNA and DNA in HBV virus-infected HepG2-NTCP cells. HepG2-NTCP cells were either mock infected ( A ) or infected with 100 × concentrated HepAD38 cell supernatant ( B – D ). Seven days after infection, cells were fixed and permeabilized, followed by hybridization with Probe set 2 and Probe set 3. After bDNA amplification, fluorophore-conjugated label probe was applied and images were acquired using a Deltavision epifluorescence microscope with a × 100 oil-immersion objective. Image ( D ) was an enlarged area in the rectangle region of ( C ). Scale bar, 4 μm.

    Article Snippet: The ViewRNA technology uses the bDNA signal amplification scheme, which was initially developed by the Chiron Corporation to quantify the HIV and HCV viral loads., , , The bDNA scheme can also be used to quantify DNA molecules, such as HBV DNA, with a detection limit of 2000 copies.

    Techniques: Virus, Infection, Hybridization, Amplification, Microscopy